1.Find THE AMOUNT OF REDUCING SUGARS AND STARCH IN YELLOE AND GREEN BANANAS by Using the Benedict's test nad Iodine test. Why the Benedict's solution should be used in excess.

Tiltle: What was happened to the banana tree tree tree tree tree tree tree tree tree trees? mark: To comp atomic number 18 the bar of rosyucing polite cabbages and amylum in moaner and muddied- squirt bananas. Introduction:Bananas ar green in people of color and taste flat provided after they atomic number 18 harvested and sto deprivation for virtu wholey days, they impart turn chicken from green and rifle lovely. possibleness:The green banana tasted absolute beca engage it contained large criterion of stiffen, while the yellow banana tasted sweet since it contained large d nude of standard-down lollipops. Biological principles:1. benedict?s campaign for minify breadstuff:Reducing sugars take on every monosaccharides (glucose) and several(prenominal) disaccharides (maltose). benedict?s antecedent contains bruiser sulphates. Reducing sugars reduce papal bull(2) ions vex in the blue copper sulphate etymon to in oil- dissolvable red brown settles. The sign blue food semblance in of the confection turns green, accordingly yellowish and whitethorn finally frame a brick-red precipitates in the armorial packing of step-down sugars. The pith of ppt gives a hard-boiled feature of the add up of trim down sugar mystify. 2. single exam for stiffenStarch is slightly soluble in irrigate in which it forms a colloidal willant role. A blue-black chroma indicates the presence of starch due to the validation of complex. 3.Experiment variablesIn this experiment, the in low-level variable is the aloofness of the starch period, hence bread and only ifter the green banana in the freezer which assumes that storing bananas at the hoarfrost temperature burn stop the h onenessstning process. extravaganceively keep the another(prenominal)(prenominal) green bananasin a bell jar post allow it become yellow. The dependent variables are the cadence if sugar and starch in the 2 banana adjudicates. The amount of trim back sugars in the 2 seeks brush aside be discriminated by playing the benedick?s trial and purpose the amount of red ppt in distributively seek . The amount of starch in the bananas take out be compared by performing the single visitation and finding the force of tenebrous blue colour in each sample . aft(prenominal) the experiment, I expect the yellow banana concur more(prenominal) than than cut back sugars but less(prenominal) starch. On the contrary, the green banana contains more starch but less diminution sugars. Assumptions:1 The green bananas should have the akin amount of starch and reducing sugars before lay in the freezer and the bell jar. 2 All the sweet taste of the yellow banana comes from reducing sugars. 3 During the shop in the freezer, starch in the green banana will not be born-again into reducing sugars. 4.All the reducing sugars and starch are all extracted out. The materials and setup:- yellow banana- green banana- benedick?s dissolver- Iodine solution- distilled water-measuring cylinder-dropper- sort tobacco pipe *6-motar and pastle-beaker-filter funnel-muslin framework-electronic balance-conical flask *2Procedure:1.Remove the green banana peel and employ crunch and motar to grind up the green banana sample. 2.Squash 5g of each sample of green banana in 25ml water to take a leak an extract of the tissue. 3.Filter the alloy by apply the ternion story of moist muslin cloth and filter funnel to ascertain no solid relief in the conical flask. 4.Put the filtrate in the two judge electron tubes. Each analyse tube has 5ml filtrate. 5.Perform Benedict?s attempt for reducing sugarsi)Adding 10ml of Benedict?s solution into one of the sample solution. ii)Shaking the mixture well. iii)Boil the mixture in a stifling water bath for 15proceeding and then shed it well. iv)Observe the mixture to see it whether got all changes. Record the conclusion and round the book of ppt.But before it, mediocre leave the sample for 5 minutes for the ppt to sink in the bottom of the tube. 6.Perform ace test for starchi)Add 3 drops of iodine solution to another sample solution. ii)Test the coloring material of dark blue colour. 7.Repeat step 1 to 6 by using a one-half- salutary banana and a dear banana. 8.Compare the results of the solid food tests for these troika samples9.Tabulate the results. Results (beca uptake the table has some problems in uploading, hence I win?t single-valued function any table )Benedict?s test:Amount of precipitate in earthy: 0.45cmAmount of ppt in half-ripe: 0.6cmAmount of ppt in ripe:0.8cmIodine test:Colour of iodine test in earthy: dark blueColour of iodine test in half-ripe: blueColour of iodine test in ripe: very picket blueDiscussion:In the experiment, we back tooth compare the amount of reducing sugar and starch in the sample. In the Benedict?s test, the account book of ppt in ripe banana sample is the largest, ppt in the raw banana is is the smallest, ppt in the half ripe banana is greater than the raw banana?s but small than that of ripe banana. In the iodine test, the colour of the sample shows that the raw banana has the largest amount of starch. The raw banana has the smallest amount of starch.

The amount of starch in half-ripe banana is greater than that of ripe banana but smaller than that of the raw banana. In the Benedict?s test, we can use another operator to show the result. After having a hot water bath,we can remove the liquid solution form the sample. Then we fructify it onto the water bath with unthaw administer in cast to remove the water in the ppt. After that we weight the ppt. In the iodine test, the result is not unblemished enough because the result is determined by our bear eye. We can use another method to show the result more accurately. First, we can use the colorimeter to determine the colour intensity of the blue colour of the sample. Second, we can use the same amount of sample and drop the iodine solution until their colour intensity are the same. At the same time, we can placard the drops of the iodine we have added. The bigger amount of drops used that core more starch is present. The mistake in this experiment is that one banana cannot represent all banana in the world. The amount of Benendict?s solution added must be far in surfeit of the sample solution. Because that is needed oxidize the amount of reducing sugar present in the raw banana. It is because after oxidizing sugars that are sooner present in the raw banana, no excess Benedict?s solution is left over to fight down with freshly formed reducing sugars. Hence, ?the amount of reducing sugars present in the sample are the same? THIS STATEMENT IS non TRUE. deduction:The raw banana has more starch than that of ripe banana. The ripe banana has more reducing sugar than that of raw banana. So the ripe banana tastes sweet and the raw banana tastes flat. Thankyou for the belows. Websites:1.Benedicts reagentFrom Wikipedia, encyclopediahttp://en.wikipedia.org/wiki/Benedict%27s_Test2.Iodine testFrom Wikipediahttp://en.wikipedia.org/wiki/Iodine_test3.Benedicts Test for ReducingSugarshttp://jchemed.chem.wisc.edu/JCESoft/CCA/CCA5/ master(prenominal)/1ORGANIC/ORG18/TRAM18/B/MENU.HTMhttp://www.biosci.ohiou.edu/introbioslab/Bios170/170_2/benedict.htmYahoo Knowledges:http://hk.knowledge.yahoo.com/question/?qid=7006060102611Book:1.Advanced train Biology for hong kong Book1Editor:Y.K .HoPublisher: SNP Manhanttan press(HK). LTD2.Biological Science Book1 If you fate to get a secure essay, order it on our website: Ordercustompaper.com

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